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Detection technologies for RNA modifications

  • 작성자

    Xiaoyu Li
  • 작성일자

    2022-11-15
  • 조회수

    376
Xiaoyu Li( xiaoyu_li@zju.edu.cn )
2020-present Assistant Professor, School of Medicine, Zhejiang University, China
2017-2020 Postdoctoral fellow, School of Life Sciences, Peking University, China
2011-2017 Ph.D., School of Life Sciences, Peking University, China
2008-2011 B.S., College of Biological Sciences, China Agricultural University, China

Detection technologies for RNA modifications

To date, more than 170 chemical modifications have been characterized in RNA, providing a new layer of gene expression regulation termed the 'epitranscriptome'. RNA modification detection methods and tools advance the functional studies of the epitranscriptome. According to the detection throughput and principles, existing RNA modification detection technologies can be categorized into four classes, including quantification methods, locus-specific detection methods, next-generation sequencing-based detection technologies and nanopore direct RNA sequencing-based technologies. In this review, we summarize the current knowledge about these RNA modification detection technologies and discuss the challenges for the existing detection tools, providing information for a comprehensive understanding of the epitranscriptome.

Exp Mol Med. 2022 Oct;54(10):1601-1616. doi: 10.1038/s12276-022-00821-0.
https://pubmed.ncbi.nlm.nih.gov/36266445/