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BMB Reports

Detecting DNA hydroxymethylation: exploring its role in genome regulation

  • 작성자

    SUN-MIN LEE
  • 작성일자

    2024-03-22
  • 조회수

    1187
Name: SUN-MIN LEE ( sml67@konkuk.ac.kr )
2022-presentBrain-Pool fellow, Department of Physics, KONKUK University, Korea
2016-2022Research associate, Gurdon Institute, Cambridge University, UK
2014-2016Postdoctoral research fellow, Yonsei University, Korea
2006-2014Ph.D., Department of Biochemistry, Yonsei University, Korea

Detecting DNA hydroxymethylation: exploring its role in genome regulation

DNA methylation is one of the most extensively studied epigenetic regulatory mechanisms, known to play crucial roles in various organisms. It has been implicated in the regulation of gene expression and chromatin changes, ranging from global alterations during cell state transitions to locus-specific modifications. 5-hydroxymethylcytosine (5hmC) is produced by a major oxidation, from 5-methylcytosine (5mC), catalyzed by the ten eleven translocation (TET) enzymes, and is gradually being recognized for its significant role in genome regulation. With the development of state-of-the-art experimental techniques, it has become possible to detect and distinguish 5mC and 5hmC at base resolution. Various techniques have evolved, encompassing chemical and enzymatic approaches, as well as thirdgeneration sequencing techniques. These advancements have paved the way for a thorough exploration of the role of 5hmC across a diverse array of cell types, from embryonic stem cells (ESCs) to various differentiated cells. This review aims to comprehensively report on recent techniques and discuss the emerging roles of 5hmC.


BMB Rep. 2024 Mar 7:6164. Online ahead of print.
https://pubmed.ncbi.nlm.nih.gov/38449301/