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BMB Reports

Harnessing CRISPR-Cas adaptation for RNA recording and beyond

  • 작성자

    Sungchul Kim
  • 작성일자

    2023-12-21
  • 조회수

    1608
Name: Sungchul Kim ( sungchulkim.kr@gmail.com )
2021-presentYoung Scientist Fellow/Principal research investigator, Center for RNA research, Institute for Basic Science, Seoul National University, Korea
2016-2021Postdoctoral researcher, Department of Bioanoscience, Faculty of Applied Science, Delft University of Technology, the Netherlands
2013-2016Postdoctoral researcher, School of Biological Sciences, Seoul National University, Korea
2007-2013Ph.D., School of Biological Sciences, Seoul National University, Korea

Harnessing CRISPR-Cas adaptation for RNA recording and beyond

Prokaryotes encode clustered regularly interspaced short palindromic repeat (CRISPR) arrays and CRISPR-associated (Cas) genes as an adaptive immune machinery. CRISPR-Cas systems effectively protect hosts from the invasion of foreign enemies, such as bacteriophages and plasmids. During a process called 'adaptation', non-self-nucleic acid fragments are acquired as spacers between repeats in the host CRISPR array, to establish immunological memory. The highly conserved Cas1-Cas2 complexes function as molecular recorders to integrate spacers in a time course manner, which can subsequently be expressed as crRNAs complexed with Cas effector proteins for the RNA-guided interference pathways. In some of the RNA-targeting type III systems, Cas1 proteins are fused with reverse transcriptase (RT), indicating that RT-Cas1-Cas2 complexes can acquire RNA transcripts for spacer acquisition. In this review, we summarize current studies that focus on the molecular structure and function of the RT-fused Cas1-Cas2 integrase, and its potential applications as a directional RNA-recording tool in cells. Furthermore, we highlight outstanding questions for RT-Cas1-Cas2 studies and future directions for RNA-recording CRISPR technologies.


BMB Rep. 2023 Dec 6:5906. Online ahead of print.
https://pubmed.ncbi.nlm.nih.gov/38053290/