생화학분자생물학회

	Name: Jong-Chan Park ( jongchan@g.skku.edu )
	2023-Current	Assistant Professor, Department of Biophysics, Sungkyunkwan University, South Korea
	2020-2022	Postdoctoral Research Fellow, University College London, UK
	2013-2019	Ph.D., Seoul National University College of Medicine, South Korea
	2008-2013	B.S., College of Life Sciences and Biotechnology, Korea University, South Korea

Electrophysiological insights with brain organoid models: a brief review

Brain organoid is a three-dimensional (3D) tissue derived from stem cells such as induced pluripotent stem cells (iPSCs) or embryonic stem cells (ESCs) that reflect real human brain structure. It replicates the complexity and development of the human brain, enabling the study of the human brain in vitro. With emerging technologies, its application is various including disease modeling and drug screening. A variety of experimental methods have been used to study the structural and molecular characteristics of brain organoids; however, electrophysiological analysis is necessary to understand their functional characteristics and complexity. Although electrophysiological approaches have rapidly advanced for monolayered cells, there are some limitations in studying the electrophysiological and neural network characteristics due to the lack of 3D characteristics. Herein, we review electrophysiological measurement and analytical methods related to the neural complexity and 3D characteristics of brain organoids. Overall, the electrophysiological understanding of brain organoids allow us to overcome the limitations of monolayer in-vitro cell culture models, providing deep insights into the complicated neural networks of the real human brain and new ways of disease modeling.


BMB Rep. 2024 Jun 26:6262. Online ahead of print.
https://pubmed.ncbi.nlm.nih.gov/38919012/