생화학분자생물학회입니다.
Cryogenic Single-molecule Fluorescence Imaging
작성자
Phil Sang Yu작성일자
2024-12-26조회수
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Name: Phil Sang Yu ( fx0908@postech.ac.kr ) | |
2017-present | Ph.D. Candidate, Single-molecule Biophysics Lab., Department of Physics, Pohang University of Science and Technology (POSTECH) | |
2011-2017 | B.S, Department of electronic physics, Hankuk University of Foreign Studies | |
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Name: Chae Un Kim ( cukim@unist.ac.kr ) | |
2018-present | Associate Professor, Department of Physics, UNIST, Korea | |
2014-2018 | Assistant Professor, Department of Physics, UNIST, Korea | |
2009-2014 | Staff Scientist, Cornell University, USA | |
2002-2008 | Ph.D., Biophysics, Cornell University, USA | |
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Name: Jong-Bong Lee ( jblee@postech.ac.kr ) | |
2011-present | Associate Professor and Professor, Department of Physics and School of Interdisciplinary Bioscience & Bioengineering, POSTECH, Korea | |
2007-2011 | Assistant Professor, Department of Physics, POSTECH, Korea | |
2004-2007 | Postdoctoral research fellow, Harvard Medical School, USA | |
1999-2004 | Ph.D., Department of Physics, Brandeis University, USA |
Cryogenic Single-molecule Fluorescence Imaging
Cryo-fixation techniques, including cryo-electron and cryo-fluorescence microscopy, enable the preservation of biological samples in a near-native state by rapidly freezing them into an amorphous ice phase. These methods prevent the structural distortions often caused by chemical fixation, allowing for high-resolution imaging. At low temperatures, fluorophores exhibit improved properties, such as extended fluorescence lifetimes, reduced photobleaching, and enhanced signal-to-noise ratios, making single-molecule imaging more accurate and insightful. Despite these advantages, challenges remain, including limitations in numerical aperture of objectives and cryo-stage for single-molecule imaging, which can affect photon detection and spatial resolution. Recent advancements at low temperatures have mitigated these issues, achieving resolutions at the nanometer scale. Looking forward, innovations in super-resolution techniques, optimized fluorophores, and Artificial Intelligence (AI)-based data analysis promise to further advance the field, providing deeper insights into biomolecular dynamics and interactions. In this mini-review, we will introduce low-temperature single-molecule fluorescence imaging techniques and discuss future perspectives in this field.
BMB Rep. 2024 Dec 20. pii: 6385. [Epub ahead of print]
https://pubmed.ncbi.nlm.nih.gov/39701023/