생화학분자생물학회

	Name: Kyungoh Jung ( kojung@cau.ac.kr )
	2021-present	Assistant Professor, Department of Anatomy, College of Medicine, Chung-Ang University
	2016-2021	Postdoctoral Fellow, Stanford University School of Medicine, USA
	2010-2016	Ph.D., Department of Biomedical Sciences, College of Medicine, Seoul National University, Republic of Korea

In Vivo Molecular Imaging and Single Cell Imaging

Molecular imaging could be used to improve the disease diagnosis, prognosis, monitoring of treatment in living tissues. Numerous molecular targets have been developed for various cellular and molecular processes in genetic, metabolic, proteomic, and cellular biologic level. Molcular imaging modalities such as Magnetic Resonance Imaging (MRI), Positron Emission Tomography (PET), Single Photon Emission Computed Tomography (SPECT), Computed Tomography (CT), and Optical Imaging can be used to visualize anatomic, genectic, biochemical, and physiologic changes in vivo. For in vivo cell imaging, certain cells such as cancer cells, immune cells, stem cells could be labeled by direct and indirect labeling methods to monitor cell migration, cell activity, and cell effects in cell-based therapy. In case of cancer, it could be used to investigate biological processes such as cancer metastasis and to analyze the drug treatment process. In addition, tranplanted stem cells and immune cells in cell-based therapy could be visualized and tracked to confirm the fate, activity, and function of cells. In conventional molecular imaging, cells can be monitored in vivo in bulk non-invasively with optical imaging, MRI, PET, and SPECT imaging. However, single cell imaging in vivo has been a great challenge due to an extremely high sensitive detection of single cell. Recently, there has been great attention for in vivo single cell imaging due to the development of sigle cell study. In vivo single imaging could analyze the survival or death, movement direction, and characteristics of a single cell in live subjects. In this paper, we reviewed the basic principles of in vivo molecular imaging and introduced recent studies for in vivo single cell imaging based on the concept of in vivo molecular imaging.


BMB Rep. 2022 Jun 2;5581. Online ahead of print.
https://pubmed.ncbi.nlm.nih.gov/35651326/