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BmB Reports

Single-molecule studies of repair proteins in base excision repair

  • 작성자

    Gwangrog Lee
  • 작성일자

    2024-12-26
  • 조회수

    478
Name: Gwangrog Lee ( ifglee@kaist.ac.kr )
2023-presentProfessor, Department of Biological Science, KAIST
2012-2023Professor, Life Science, GIST
2006-2012Postdoctoral research fellow, UIUC, IL, USA
2006-2006Post-doctoral fellow, Duke University, Durham, NC, USA
2002-2006Ph.D. MEMS, Duke University, Durham, NC, USA

Single-molecule studies of repair proteins in base excision repair

Base excision repair (BER) is an essential cellular mechanism that repairs small, non-helix-distorting base lesions in DNA, resulting from oxidative damage, alkylation, deamination, or hydrolysis. This review highlights recent advances in understanding the molecular mechanisms of BER enzymes through single-molecule studies. We discuss the roles of DNA glycosylases in lesion recognition and excision, with a focus on facilitated diffusion mechanisms such as sliding and hopping that enable efficient genome scanning. The dynamics of apurinic/apyrimidinic endonucleases, especially the coordination between APE1 and DNA polymerase β (Pol β), are explored to demonstrate their crucial roles in processing abasic sites. The review further explores the short-patch and long-patch BER pathways, emphasizing the activities of Pol β, XRCC1, PARP1, FEN1, and PCNA in supporting repair synthesis and ligation. Additionally, we highlight the emerging role of UV-DDB as a general damage sensor in BER, extending its recognized function beyond nucleotide excision repair. Single-molecule techniques have been instrumental in uncovering the complex interactions and mechanisms of BER proteins, offering unprecedented insights that could guide future therapeutic strategies for maintaining genomic stability.


BMB Rep. 2024 Dec 20. pii: 6383. [Epub ahead of print]
https://pubmed.ncbi.nlm.nih.gov/39701025/